| Postdoctoral | Graduate | Undergraduate |
None available at the moment.
PhD position in polar bear landscape genomics.
We recently received $9.2 million CDN funding for a pan-Arctic polar bear project from Genome Canada and associated co-funders – title: BEARWATCH: Monitoring Impacts of Arctic Climate Change using Polar Bears, Genomics and Traditional Ecological Knowledge. The Public Summary of the project can be found | Here |.
As part of this project, we will survey genomic variation among polar bear populations across the Canadian Arctic. The survey will encompass the majority of the global polar bear population and draw upon an unprecedented repository of over 7,000 tissue samples spanning the NWT and Nunavut, and > two decades. The basis of this doctoral project will be the exploration of spatial and temporal patterns of genetic connectivity/gene flow in the face of unprecedented Arctic environmental change and diminishing sea ice using genome-wide panels of Single Nucleotide Polymorphisms (SNPs), and novel landscape and evolutionary genomics approaches, with lots of scope for creativity and novel research questions.
The Department of Biology at Queen’s University (http://biology.queensu.ca/) is dynamic and collaborative department with 36 tenured or tenure-track faculty, well-equipped molecular and microscopy facilities, multiple well-attended seminar series, and one of the best field stations in Canada (http://www.queensu.ca/qubs/). Queen’s University is located in the centre of the small, historic city of Kingston, Ontario, on the northern shore of Lake Ontario.
• M.Sc. in biology or related discipline preferably with some knowledge of genetics or genomics or B.Sc. with first class standing with similar background.
Skills & experience:
• strong interpersonal and communication skills
• excellent oral and written English communication skills
• strong academic track record
• familiarity with theory and applications in evolutionary and population genetics/genomics
• familiarity with statistics and data analysis
Additional desirable experience:
• some experience with ddRAD-Seq or Genotyping-by-Sequencing data or other Next Gen Sequencing methods
• familiarity with GIS analytical tools
• experience with processing Next Gen Sequencing data
Note that we will provide training in all of these.
Stipend and research support for 4 years with terrific opportunities to develop teaching and mentorship skills, practice grant-writing, and undertake some interesting side projects in conservation and landscape genomics.
Interested applicants should contact Stephen Lougheed (firstname.lastname@example.org) directly. Please include 1) your CV that highlights the relevant skills, 2) a one-paragraph summary of your career goals and why you would like to undertake a PhD at Queen’s, 3) names of three references and their contact information. Start: January 2018.
Prospective Undergraduate Thesis Students
We have filled all positions for our lab for the 2017-18 school year. Below we list some areas of research that have been the focus of past undergraduate theses.
1. Fish genomics
Central to such ecologically-sound and sustainable fishing practices, is an understanding of the stock populations and their genetic and adaptive differences. Failure to manage fisheries on good science can have catastrophic consequences most obviously evidenced in recent years by the collapse of the east coast Atlantic cod fisheries. Dr. Lougheed and colleagues were recently awarded funding from Genome Canada to use leading-edge genomics and bioinformatics to define fish stocks (http://arcticfishery.ca/). We will map and interpret the genomic diversity of wild Lower Northwest Passage populations of Arctic char, Arctic cod, and a shrimp species, taxa that have the most promise for a managed fishery. The goal is to sequence the genomes of focal species and to develop species-specific single nucleotide polymorphism (SNPs) using next generation sequencing methods, work that will be undertaken by graduate students, a postdoctoral fellow, and an undergraduate summer student. These genomic data will be integrated with traditional ecological knowledge (TEK) to develop a sustainable management of these emerging fisheries, facilitating the development of commercial opportunities, increasing employment, providing a healthy food source and food security, and contributing to increased prosperity and well-being for the people of Nunavut. This project would focus on quantifying patterns of differentiation among different sampled char populations and interpreting their recent evolutionary and population dynamics.
2. Frog speciation
One of the most enduring goals in evolutionary biology is to understand how new species arise. The traditional view, popularized by 20th Century evolutionary biologist Ernst Mayr, emphasized a geographic view of speciation where most new species arise or at least begin through physical and thus genetic isolation of ancestral populations. Recent theoretical and empirical work has moved away from these geography-based views of speciation to ones that emphasize mechanism (e.g. selection driving ecological divergence) producing important insights into how new species arise. However, our own research over the last 5 years highlights the pervasiveness of both geographic isolation and subsequent secondary contact in the histories of most vertebrate species, mirroring comprehensive reviews of the phylogeographic literature. Our NSERC-funded research focuses on a single temperate North American frog, the spring peeper (Pseudacris crucifer). Our published work suggests that the spring peeper has a dynamic evolutionary history with 6 well-supported evolutionary lineages that originated in distinct, isolated refugia between 11 and 3 million years before present. Different lineage pairs with disparate times of divergence have come into secondary contact in different parts of the species’ range, presumably within the last 10-15,000 years. We are tackling a series of questions one of which might help frame a 537 project: (i) What is the relation of divergence times between evolutionary lineages and patterns of reproductive isolation, the hallmark of biological species? (ii) What do genomic data say about the patterns of gene flow among lineages over the entire history of this species? (iii) Does secondary contact enhance reproductive barriers of closely-related lineages, either to diminish acoustic interference of male advertisement calls used by females in mate selection, or to prevent maladaptive hybridization? (iv) How might marked differences in the acoustic environment or seasonality shape the outcomes of secondary contact between diverging lineages? (v) What are the various fitness costs of hybridization between lineages?
3. Reptile landscape genetics
Landscape genetics and increasingly genomics have proved indispensible in augmenting our understanding of how physical and biotic environmental features modulate dispersal, gene flow, and local adaptation. We have embraced these approaches combining molecular surveys, GIS and spatially-explicit simulations, and detailed radiotelemetry and demographic studies. Our Ontario work focuses on reptiles of conservation concern, evaluating the impact of habitat fragmentation on population connectivity and persistence. This work collectively shows far greater genetic subdivision within the distributions of focal Canadian species than would be predicted based on apparent distribution, and implies that habitat loss and degradation threaten their persistence over much if their respective ranges. We have assembled samples from a range of species-at-risk snakes from across Ontario but focused on southwestern Ontario and the eastern shores of Georgian Bay, and are working to quantify patterns of diversity and genetic differentiation as they relate to past and present landscape usage and fragmentation. The ultimate goal is to both understand how human-induced changes to habitat have impacted the probability of species persistence and how we might mitigate these impacts.
4. Grassland bird habitat modeling
Habitat loss and degradation are two of the most significant and widespread factors contributing to species declines and extinction. In North America, native prairie and grassland habitats have suffered some of the greatest losses – less than 1% of tallgrass prairie remains, and mixed-grass and short-grass prairie have been reduced to 20-30% of their former extent. Fifty-seven percent of North American grassland bird species are undergoing significant, long-term population declines. Thus far, conservation efforts have focused primarily on breeding habitat, but they have not been able to reverse population declines. The role of threats faced during the wintering season is believed to be important, but it has received relatively little attention. Our lab has been working to quantify migratory connectivity and develop a full annual life cycle model for the Loggerhead Shrike, a species associated with grassland habitat, has experienced the 6th most drastic population decline of any landbird species over the life of the Breeding Bird Survey. We have amassed wintering season territory locational data for more than 900 shrikes. Each bird has been distinguished as migrant or resident using stable isotopes and genetic data. This massive data set will be used to develop a spatially explicit model of winter habitat use for Loggerhead Shrike. The ultimate goal is to help the North American Loggerhead Shrike Working Group develop standardized methodology that can be applied across the species range and used as the basis of further study of winter season demographics and limiting factors.